Journal of Diabetes and Its Complications
Volume 25, Issue 2 , Pages 114-121 , March 2011

Caldesmon over-expression in type 1 diabetic nephropathy

  • Renato Millioni

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
  • ,
  • Elisabetta Iori

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
  • ,
  • Livia Lenzini

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
  • ,
  • Lucia Puricelli

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
  • ,
  • Brasilina Caroccia

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
  • ,
  • Giorgio Arrigoni

      Affiliations

    • Department of Biological Chemistry, University of Padova, Italy
    • VIMM, Venetian Institute of Molecular Medicine, Padova, Italy
  • ,
  • Gian Paolo Rossi

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
  • ,
  • Paolo Tessari

      Affiliations

    • Department of Clinical and Experimental Medicine, University of Padova, Italy
    • Corresponding Author InformationCorresponding author. Department of Clinical and Experimental Medicine, University of Padova, via Giustiniani 2, 35128 Padova, Italy. Tel.: +39 0498211748; fax: +39 0498754179.

Received 28 December 2009 ,Revised 2 July 2010 ,Accepted 13 July 2010.

References 

  1. Anderson L, Seilhamer J. A comparison of selected mRNA and protein abundances in human liver. Electrophoresis. 1997;18:533–537
  2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976;72:248–254
  3. Ceriello A, Morocutti A, Mercuri F, Quagliaro L, Moro M, Damante G, et al. Detective intracellular antioxidant enzyme production in type 1 diabetic patients with nephropathy. Diabetes. 2000;49:2170–2177
  4. Clarkson MR, Murphy M, Gupta S, Lambe T, Mackenzie HS, Godson C, et al. High glucose-altered gene expression in mesangial cells. Actin-regulatory protein gene expression is triggered by oxidative stress and cytoskeletal disassembly. Journal of Biological Chemistry. 2002;277:9707–9712
  5. Conway BR, Maxwell AP, Savage DA, Patterson CC, Doran PP, Murphy M, et al. Association between variation in the actin-binding gene caldesmon and diabetic nephropathy in type 1 diabetes. Diabetes. 2004;53:1162–1165
  6. Cortes P, Mendez M, Riser BL, Guerin CJ, Rodriguez-Barbero A, Hassett C, et al. F-actin fibre distribution in glomerular cells: Structural and functional implications. Kidney International. 2000;58:2452–2461
  7. Dominguez DC, Lopes R, Torres ML. Proteomics technology. Clinical Laboratory Science. 2007;20(4):239–244
  8. Fogarty DG, Moczulski DK, Makita Y, Araki S, Rogus JJ, Warram JH, et al. Evidence for susceptibility locus to diabetic nephropathy on chromosome 7q in Caucasian families with type 2 diabetes. Diabetes. 1999;48(Suppl. 1):A47
  9. Futcher B, Latter GI, Monardo P, McLaughlin CS, Garrels JI. A sampling of the yeast proteome. Molecular Biology of the Cell. 1999;19:7357–7368
  10. Gharahdaghi F, Weinberg CR, Meagher DA, Imai BS, Mische SM. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis. 1999;20:601–605
  11. Gygi SP, Rochon Y, Franza BR, Aebersold R. Correlation between protein and mRNA abundance in yeast. Molecular Biology of the Cell. 1999;19:1720–1730
  12. Helfman DM, Levy ET, Berthier C, Shtutman M, Riveline D, Grosheva I, et al. Caldesmon inhibits nonmuscle cell contractility and interferes with the formation of focal adhesions. Molecular Biology of the Cell. 1999;10:3097–3112
  13. Huang C, Kim Y, Caramori ML, Fish AJ, Rich SS, Miller ME, et al. Cellular basis of diabetic nephropathy: II. The TGF-b system and diabetic nephropathy lesions in type 1 diabetes. Diabetes. 2002;51:3577–3581
  14. Huber PA. Caldesmon. The International Journal of Biochemistry & Cell Biology. 1998;29:1047–1051
  15. Imperatore G, Hanson RL, Pettitt DJ, Kobes S, Bennett PH, Knowler WC. Pima Diabetes Genes Group: Sib-pair linkage analysis for susceptibility genes for microvascular complication among Pima Indians with type 2 diabetes. Diabetes. 1998;47:821–830
  16. Iori E, Marescotti MC, Vedovato M, Ceolotto G, Avogaro A, Tiengo A, et al. In situ protein kinase C activity is increased in cultured fibroblasts from type 1 diabetic patients with nephropathy. Diabetologia. 2003;46:524–530
  17. Iori E, Millioni R, Puricelli L, Arrigoni G, Lenzini L, Trevisan R, et al. Glycolytic enzyme expression and pyruvate kinase activity in cultured fibroblasts from type 1 diabetic patients with and without nephropathy. Biochimica and Biophysica Acta. 2008;1782:627–633
  18. Lurbe A, Fioretto P, Mauer M, LaPointe MS, Batlle D. Growth phenotype of cultured skin fibroblasts from IDDM patients with and without nephropathy and overactivity of the Na+/H+ antiporter. Kidney International. 1996;50:1684–1693
  19. Matsumura F, Yamashiro S. Caldesmon. Current Opinion in Cell Biology. 1993;5:70–76
  20. Millioni R, Iori E, Puricelli L, Arrigoni G, Vedovato M, Trevisan R, et al. Abnormal cytoskeletal protein expression in cultured skin fibroblasts form type 1 diabetes mellitus patients with nephropathy: A proteomic approach. Proteomics Clinical Applications. 2008;2:492–503
  21. Perez JF, Reeds PJ. A new stable isotope method enables the simultaneous measurement of nucleic acid and protein synthesis in vivo in mice. Journal of Nutrition. 1998;128:1562–1569
  22. Podestà F, Meregalli G, Ghelardi R, Del Giudice R, Asnaghi V, Maestroni A, et al. Low Ca2+ pump activity in diabetic nephropathy. American Journal of Kidney Disease. 2001;38:465–472
  23. Puricelli L, Iori E, Millioni R, Arrigoni G, James P, Vedovato M, et al. Proteome analysis of cultured fibroblasts from type 1 diabetic patients and normal subjects. Journal of Clinical and Endocrinology Metabolism. 2006;91:3507–3514
  24. Saalbach A, Aneregg U, Bruns M, Schnabel E, Herrmann K, Haustein UF. Novel fibroblast-specific monoclonal antibodies: Properties and specificities. Journal of Investigative Dermatology. 1996;106(6):1314–1319
  25. Saalbach A, Aust G, Haustein UF, Herrmann K, Anderegg U. The fibroblast-specific MAb AS02: A novel tool for detection and elimination of human fibroblasts. Cell and Tissue Research. 1997;290(3):593–599
  26. Schena FP, Gesualdo L. Pathogenetic mechanisms of diabetic nephropathy. Journal of American Society of Nephrology. 2005;16:30–33
  27. Tessari P, Puricelli L, Iori E, Arrigoni G, Vedovato M, James P, et al. Altered chaperone and protein turnover regulators expression in cultured skin fibroblasts from type 1 diabetes mellitus with nephropathy. Journal of Proteome Research. 2007;6:976–986
  28. Trevisan R, Fioretto P, Barbosa J, Mauer M. Insulin-dependent diabetic sibling pairs are concordant for sodium hydrogen antiport activity. Kidney International. 1999;55:2383–2389
  29. Trevisan R, Li LK, Messent J, Tariq T, Earle K, Walzer JD, et al. Na+/H+ antiport activity and cell growth in cultured skin fibroblasts of IDDM patients with nephropathy. Diabetes. 1992;41:1239–1246
  30. UK Prospective Diabetes Study (UKPDS) Group. The Diabetes Control and Complications Trial Research Group: The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus. New England Journal of Medicine. 1993;329:977–986
  31. UK Prospective Diabetes Study (UKPDS) Group. Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes. Lancet. 1998;352:837–853
  32. Yamashiro S, Chern H, Yamakita Y, Matsumura F. Mutant caldesmon lacking cdc2 phosphorylation sites delays M-phase entry and inhibits cytokinesis. Molecular Biology of the Cell. 2001;12:239–250
  33. Zatz R, Brenner BM. Pathogenesis of diabetic microangiopathy: The haemodynamic view. American Journal of Medicine. 1986;80:443–453
  34. Zhou X, Hurst RD, Templeton D, Whiteside CI. High glucose alters actin assembly in glomerular mesangial and epithelial cells. Laboratory Investigation. 1995;73:372–383

 Disclosure: All the authors declared no competing interests.

PII: S1056-8727(10)00076-0

doi: 10.1016/j.jdiacomp.2010.07.005

Journal of Diabetes and Its Complications
Volume 25, Issue 2 , Pages 114-121 , March 2011