Mutation H63D in the HFE gene confers risk for the development of type 2 diabetes mellitus but not for chronic complications☆☆☆
Received 22 June 2009; received in revised form 1 December 2009; accepted 10 December 2009. published online 25 January 2010. Corrected Proof
Abstract
Purpose
To evaluate the frequency of mutations in the HFE gene (C282Y and H63D) in type 2 diabetes mellitus (DM) patients and their possible association with diabetic chronic complications.
Methods
A case-control study with 723 subjects was performed. All diabetic subjects (n=519) underwent a clinical and laboratory evaluation. Diabetic retinopathy (DR) was evaluated by an ophthalmologist. Diabetic nephropathy (DN) was categorized by urinary albumin excretion (UAE) as normoalbuminuria (n=247), microalbuminuria (n=68), macroalbuminuria (n=70), or the presence of end-stage renal disease (dialysis; n=134). Data available for blood donors (n=204) were limited to age, sex, body mass index, and absence of previous diagnosis of diabetes and normal fasting plasma glucose. The mutations C282Y and H63D in the HFE gene were genotyped based on PCR protocols and digested with the restriction enzymes SnabI (C282Y) and MboI (H63D).
Results
There was an association of type 2 DM with H63D polymorphism (genotypes HD/DD: OR=1.7, 95% CI=1.2–2.6), but not with C282Y polymorphism (OR=0.7, 955 CI=0.4–1.4). In respect to the chronic complications, there was no difference in the prevalence of DR, DN, or ischemic heart disease among the different genotypes.
Conclusions
Mutation H63D in the HFE gene was associated with a higher risk of type 2 DM, but did not appear to confer risk for diabetic chronic complications. The mutation C282Y was not associated with diabetes or its chronic complications.
Endocrine Division, Hospital de Clinicas de Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Corresponding author. Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, 900035 003 Porto Alegre, RS, Brazil. Tel.: +55 51 2101 8127; fax: +55 51 2101 8777.
☆ Conflict of interest: the authors do not have any conflict of interest.
☆☆ This study was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundo de Incentivo à Pesquisa (FIPE) do Hospital de Clínicas de Porto Alegre. LHC was a recipient of a grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). MLC is a recipient of a scholarship from the Brazilian Coordination for the Improvement of Higher Education Personnel.
ABSTRACT