Journal of Diabetes and Its Complications
Volume 25, Issue 1 , Pages 14-18 , January 2011

Hemoglobin Raleigh results in factitiously low hemoglobin A1c when evaluated via immunoassay analyzer

  • Nina Jain

      Affiliations

    • Division of Pediatric Endocrinology, Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    • Corresponding Author InformationCorresponding author. Division of Endocrinology, Department of Pediatrics, University of North Carolina at Chapel Hill, CB#7039, 3341 MBRB, Chapel Hill, NC 27599-7039, USA. Tel.: +1 919 966 4435x244; fax: +1 919 966 2423.
    • ,
  • Mehmet Kesimer

      Affiliations

    • Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    • ,
  • James D. Hoyer

      Affiliations

    • Laboratory of Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    • ,
  • Ali S. Calikoglu

      Affiliations

    • Division of Pediatric Endocrinology, Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

Received 3 June 2009 ,Revised 31 August 2009 ,Accepted 25 September 2009.

References 

  1. American Diabetes Association . Standards of medical care for patients with diabetes mellitus. Diabetes Care. 2003;26(Suppl. 1):S33–S50
  2. Bry L, Chen PC, Sacks DB. Effects of hemoglobin variants and chemically modified derivatives on assays for glycohemoglobin. Clinical Chemistry. 2001;47:153–163
  3. Chen D, Crimmins DL, Hsu FF, Lindberg FP, Scott MG. Hemoglobin Raleigh as the cause of a falsely increased hemoglobin A1c in an automated ion-exchange HPLC method. Clinical Chemistry. 1998;44:1296–1301
  4. Hoyer JD, Wick MJ, Thibodeau SN, Kechteiger KS, Cook JD, Fairbanks VF. Hb Silver Springs [® 131 (Ha) Glu → His ], a new hemoglobin variant found in six African-Americans. Hemoglobin. 1998;22:37–44
  5. Moriwaki Y, Yamamoto T, Shibutani Y, Harano T, Takahashi S, Hada T. Abnormal haemoglobins, Hb Takamatsu and Hb G-Szuhu, detected during the analysis of glycated haemoglobin (HbA1c) by high performance liquid chromatography. Journal of Clinical Pathology. 2000;53:854–857
  6. Patrinos GP, Giardine B, Riemer C, Miller W, Chui DH, Anagnou NP, et al. Improvements in the HbVar database of human hemoglobin variants and thalassemia mutations for population and sequence variation studies. Nucleic Acids Research. 2004;32(Database issue):D537–D541http://globin.cse.psu.edu/hbvar/menu.html
  7. Rai DK, Landin B, Griffiths WJ, Alvelius G. Identification of N-terminal acetylation in Hb Raleigh (β1Val→Ac-Ala) by electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 2002;16:1793–1796
  8. Roberts WL, Frank EL, Moulton L, Papadea C, Noffsinger JK, Ou C. Effects of nine hemoglobin variants on five glycohemoglobin methods. Clinical Chemistry. 2000;46:569–572
  9. Sacks DB. Hemoglobin variants and hemoglobin A1c analysis: problem solved?. Clinical Chemistry. 2003;49:1245–1247
  10. Schnedl WJ, Liebminger A, Roller RE, Lipp RW, Krejs GJ. Hemoglobin variants and determination of glycated hemoglobin (HbA1c). Diabetes/Metabolism Research and Reviews. 2001;17:94–98
  11. Thomas LB, Agosti SJ, Man MA, Mastorides SM. Screening for hemoglobinopathies during routine hemoglobin A1c testing using the Tosoh G7 Glycohemoglobin Analyzer. Annals of Clinical and Laboratory Science. 2007;37:251–255

PII: S1056-8727(09)00095-6

doi: 10.1016/j.jdiacomp.2009.09.004

Journal of Diabetes and Its Complications
Volume 25, Issue 1 , Pages 14-18 , January 2011

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