Effect of bovine amniotic fluid on intra-abdominal adhesion in diabetic male rats
Received 12 August 2009; accepted 10 September 2009. published online 15 October 2009. Corrected Proof
Abstract
Background
Postsurgical adhesion formation is a significant clinical problem within every surgical specialty. In type I diabetic patients, the problem is more severe and wound healing is slow. A wide variety of treatments have been proposed to deal with the problems that adhesion causes. One of the modalities that have not been studied extensively yet is the use of amniotic fluid. The purpose of the present study was to evaluate the clinical value of bovine amniotic fluid (BAF) efficacy in the treatment of postsurgical adhesion formation in diabetic male rats.
Materials and methods
Fifty male Wistar rats in five groups were used for our study, with animal identification being facilitated by a microchip implant system. Diabetes was induced in all groups except for the control group by intraperitoneal alloxan injection (120 mg/kg). Based upon blood glucose concentration, rats received either one third of the required insulin (two groups) or all the required insulin (remaining groups). After 2 weeks, a laparotomy was performed on each rat and adhesions were scaled. Bovine amniotic fluid was then applied to two groups, and, as a control, sterilized water was applied to the other groups. After 2 weeks, a laparotomy was again performed on each rat and adhesion was rescored.
Results and Conclusion
Significant reductions (P<.05) in adhesions were seen with BAF only in those diabetic rats that had received the required insulin. The results of our study suggest that BAF could be effective in the treatment of adhesion formation during diabetes.
aFaculty of Veterinary Medicine, Shahrekord Azad University, Shahrekord, Iran
bDepartment of Biology, Faculty of Science, The University of Isfahan, Isfahan, Iran
cCell, Molecular and Developmental Division, Torabinejad Dental Research Centre, Dental Faculty, The University of Isfahan Medical Sciences, Isfahan, Iran
Corresponding author. Cell, Molecular and Developmental Division, Biology Department, Faculty of Science, The University of Isfahan, Isfahan, Iran. Tel.: +983117932490; fax: +983117932456.
ABSTRACT