Evaluation of the effect of oxidative stress and vitamin E supplementation on renal function in rats with streptozotocin-induced Type 1 diabetes

Haidara, Mohamed A.; Mikhailidis, Dimitri P.; Rateb, Moshira A.; Ahmed, Zeinab A.; Yassin, Hanaa Z.; Ibrahim, Ibrahim M.; Rashed, Laila A.

doi:10.1016/j.jdiacomp.2008.02.011

« Previous Next »Journal of Diabetes and Its Complications
Volume 23, Issue 2 , Pages 130-136, March 2009

Evaluation of the effect of oxidative stress and vitamin E supplementation on renal function in rats with streptozotocin-induced Type 1 diabetes

Mohamed A. Haidara
- Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Dimitri P. Mikhailidis
- Department of Clinical Biochemistry (Vascular Prevention Clinic), Royal Free Hospital, Royal Free, London, UK
- University College Medical School (University of London), London, UK
Moshira A. Rateb
- Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
- Corresponding author. Physiology Department, Faculty of Medicine, Kasr El Aini Hospital, Manial El Roda, Cairo University, Cairo, Egypt. Tel.: (202) 2516 5916; Mobile: +20 10 6224104; fax: (202) 2516 5916.
Zeinab A. Ahmed
- Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Hanaa Z. Yassin
- Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Ibrahim M. Ibrahim
- Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Laila A. Rashed
- Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt

Received 2 May 2007; received in revised form 9 February 2008; accepted 28 February 2008. published online 24 April 2008.

Abstract

We investigated the possible role of reactive oxygen species (ROS) on renal function in experimental diabetes.

Materials and Methods

Seven groups of male rats were studied. Group I consisted of control animals. Diabetes was induced (by streptozotocin) in the animals in the other groups and they received either insulin or vitamin E (300 or 600 mg/kg), both insulin and vitamin E, or no treatment for 4 weeks. At the end of the study, blood pressure was measured and parameters of kidney function and oxidative stress were evaluated in serum and kidney tissue samples.

Results

Diabetic animals had higher blood pressures; increased serum glucose, urea, creatinine, cyclic guanosine monophosphate (cGMP); increased kidney tissue levels of malondialdehyde and inducible nitric oxide synthetase (iNOS); and reduced serum glutathione peroxidase when compared with control animals. Blood glucose levels in diabetic animals were controlled by insulin and not by any dose of vitamin E alone. However, all other measured parameters improved towards control levels with either insulin or vitamin E in either dose. An additive beneficial effect was observed on the levels of iNOS and cGMP when both forms of treatment were used in diabetic animals.

Conclusions

We conclude that ROS may play an important role in diabetes-induced nephropathy in this rat model. Vitamin E supplementation in addition to insulin can have additive protective effects against deterioration of renal function in this model.

Keywords: Diabetes, Renal function, Oxidative stress, Antioxidants, Vitamin E