Increased interleukin-18 content and angiogenic activity of sera from diabetic (Type 2) patients with background retinopathy

Skopiński, Piotr; Rogala, Ewa; Duda-Król, Barbara; Lipińska, Anna; Sommer, Ewa; Chorostowska-Wynimko, Joanna; Szaflik, Jerzy; Partyka, Iwona; Skopińska-Różewska, Ewa

doi:10.1016/j.jdiacomp.2005.02.008

« Previous Next »Journal of Diabetes and Its Complications
Volume 19, Issue 6 , Pages 335-338, November 2005

Increased interleukin-18 content and angiogenic activity of sera from diabetic (Type 2) patients with background retinopathy

Piotr Skopiński
- Department of Ophthalmology, 2nd Faculty of Medicine, Warsaw Medical University, Poland
- Department of Histology and Embryology, Center of Biostructure Research, Warsaw Medical University, Poland
- Corresponding authors. Department of Ophthalmology, 2nd Faculty of Medicine, Warsaw Medical University, Poland. Tel. +48 22 643 41 46; fax: +48 22 643 41 46.
Ewa Rogala
- 3rd Department Lung Diseases, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland
Barbara Duda-Król
- 3rd Department of Internal Medicine, 2nd Faculty of Medicine, Warsaw Medical University, Poland
Anna Lipińska
- Department of Internal Medicine, Faculty of Dentistry, Warsaw Medical University, Poland
Ewa Sommer
Joanna Chorostowska-Wynimko
- Department of Laboratory Diagnostics and Immunology, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland
Jerzy Szaflik
- Department of Ophthalmology, 2nd Faculty of Medicine, Warsaw Medical University, Poland
Iwona Partyka
- Department of Ophthalmology, 2nd Faculty of Medicine, Warsaw Medical University, Poland
Ewa Skopińska-Różewska
- Department of Pathology, Warsaw Medical University, Poland
- Corresponding author. Tel/fax: +48 22 643 41 46.

Received 23 June 2004; received in revised form 7 February 2005; accepted 15 February 2005.

Abstract

Hyperglycaemia increases inflammatory cytokine concentration in the blood. Elevated levels of interleukin-18 (IL-18), a cytokine belonging to the interleukin-1 (IL-1) family, were recently reported in patients with Type 2 diabetes mellitus (DM2) and nephropathy.

The aim of the present work was an examination of IL-18 concentration in the sera of elderly DM2 patients with nonproliferative retinopathy and age-matched control people and an estimation whether this cytokine plays pro- or anti-angiogenic role in in vivo angiogenic activity of their sera in mice cutaneous angiogenesis test. Recombinant human IL-18 injected intradermally to murine skin induced significant neovascular reaction. DM2 patients sera contained higher concentration of IL-18 and induced stronger neovascular reaction in mice skin than did the sera of corresponding control people. Sera from both groups of people after neutralization with antihuman IL-18 antibodies lost substantial part of their angiogenic activity.

Keywords: Diabetes type 2, Background retinopathy, Angiogenesis, Interleukin-18